High Resolution Characterization of Proteins in Amorphous Solids

Over the last decade, our group has developed solid-state hydrogen deuterium exchange (ssHDX-MS) and photolytic labeling (ssPL-MS) with mass spectrometric analysis to probe protein conformation and matrix interactions in lyophilized solids with peptide-level resolution. This talk will describe the methods and present some recent results. The methods show promise for screening lyophilized formulations to maintain conformation and minimize aggregation, and provide insights into the properties of amorphous solids.